Composite

Part:BBa_K4644010:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-10-06)


pETlac-ureABC-lac-ureEFGD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 1726
    Illegal EcoRI site found at 2667
    Illegal XbaI site found at 806
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 3668
    Illegal PstI site found at 4296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 1726
    Illegal EcoRI site found at 2667
    Illegal NheI site found at 4520
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 3668
    Illegal PstI site found at 4296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 1726
    Illegal EcoRI site found at 2667
    Illegal BglII site found at 2876
    Illegal BamHI site found at 2424
    Illegal BamHI site found at 5641
    Illegal XhoI site found at 2533
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 1726
    Illegal EcoRI site found at 2667
    Illegal XbaI site found at 806
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 3668
    Illegal PstI site found at 4296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 1726
    Illegal EcoRI site found at 2667
    Illegal XbaI site found at 806
    Illegal SpeI site found at 2366
    Illegal SpeI site found at 3668
    Illegal PstI site found at 4296
    Illegal AgeI site found at 768
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3008


Design Notes

Using pETlac-ureABC and pETlac-ureEFGD as templates, we amplified the two expression frames separately. After connecting them, we homologously recombined them with the pETlac plasmid backbone to obtain this.

Source

Providencia rettgeri

References

Liu, X., Zhang, Q., Zhou, N. et al. Expression of an Acid Urease with Urethanase Activity in E. coli and Analysis of Urease Gene. Mol Biotechnol 59, 84–97 (2017).